Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving oxidoreductase
Patent
1988-02-10
1990-12-18
Kepplinger, Esther L.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving oxidoreductase
435 25, 435 26, 435 28, 435810, C12Q 128, C12Q 126, C12Q 100
Patent
active
049786156
ABSTRACT:
The present invention provides a method for the determination of a compound having mercapto group which comprises reacting the compound with a chromogen represented by the following general formula (I): ##STR1## wherein Y is hydrogen or hydroxyl; R.sub.1, R.sub.2 and R.sub.3 may be the same or different, and are groups represented by the following general formula (II), (III) or (IV): ##STR2## wherein Z (which may be the same or different) is hydroxyl, amino, substituted amino, alkyl, substituted alkyl, alkenyl, aryl, substituted aryl, acyl, halogen, nitro, sulfo, carboxyl or alkoxy; n is 0, 1, 2, or 3; provided that at least one Z in R.sub.1, R.sub.2 and R.sub.3 is hydroxyl, amino, or substituted amino, in the presence of peroxidase or thiol oxide reductase, and determining the pigment thus formed.
REFERENCES:
patent: 2905594 (1959-09-01), Morris
patent: 4036863 (1977-07-01), Karger
patent: 4448446 (1984-05-01), Flores
patent: 4592996 (1986-06-01), Yamanishi
patent: 4613465 (1986-09-01), Yamanishi
patent: 4673635 (1987-06-01), Yamanishi et al.
Chemical Abstracts, vol. 90, No. 23 (1979), 182296q.
Aoyama Norihito
Miike Akira
Shimizu Yoshiaki
Tatano Toshio
Kepplinger Esther L.
Kyowa Medex Co., Ltd.
Scheiner Laurie A.
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