Method of radioimmunoassay employing diffusion

Details Method of radioimmunoassay employing diffusion Method of radioimmunoassay employing diffusion

1974-01-18

1976-09-21

Padgett, Benjamin R.

Drug, bio-affecting and body treating compositions

Radionuclide or intended radionuclide containing; adjuvant...

Attached to antibody or antibody fragment or immunoglobulin;...

23230B, G01N 3300, G21H 502

Patent

active

039819817

ABSTRACT:
A method for the radioimmunoassay of small molecules consisting of bringing the large-molecule antibody of the small-molecule antigen to be determined into a slit-shaped chamber between dialysis membranes in a reaction vessel, bringing the antigen to be determined into this tank, into which a specified quantity of the radioactive form of this antigen has been added. During incubation the antigen and its radioactive form freely penetrate through the membranes and react with the antibody, whereupon washing stage is accomplished. Water and the unreacted antigen freely pass through the membranes, and the radioactivity of the washed complex remaining between the membranes is measured and the antigen is determined from this quantitatively in a way known.

REFERENCES:
kabat, Structural Concepts in Immunology and Immunochemistry, Holt, Reinhart and Winston, Inc., New York, 1968, pp. 67-81.

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