DNA strand coding for alpha-acetolactate decarboxylase and yeast

Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Preparing oxygen-containing organic compound

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4323201, 432 91, 432255, 432256, 432232, 4321723, 536 27, 426 11, 426 12, 935 56, 935 69, C12P 2100, C12C 100, C12G 100, C07H 1512

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050432761

ABSTRACT:
DNA strand having an ability in biotechnological production of .alpha.-acetolactate decarboxylase is disclosed. The DNA strand is characterized in that it has a nucleotide sequence coding for a polypeptide whose amino acid sequence is substantially from A to B of FIG. 1 and which has .alpha.-acetolactate decarboxcylase activity. Also disclosed is a yeast which belongs a Saccharomyces cerevisiae and which has been transformed by the DNA strand. The yeast is characterized by the fact that its .alpha.-acetolactate producing ability is reduced, and will thus produce an alcoholic liquor such as beer which contains no or little diacetyls which have come from their precursor, namely .alpha.-acetolactate.

REFERENCES:
patent: 4617273 (1926-10-01), Olsen
patent: 4895802 (1980-01-01), Sone et al.
"Cloning and Expression-" Goelling et al.; Applied and Environmental Microbiology 1889-1891 (1988).
"Molecular Cloning-" H. Sone; Journal of Biotechnology vol. 5, 1987.
Article "Acetolactate Decarboxylase from Aerobacter . . . " Loken et al.; Eur. J. Biochem. 14, 1970, 133-137; Article 2,3-Butanediol Biosynthetic-Stromer; Methods in Enzymology, vol. 41, pp. 518-533.
Article "Use of A-Acetolactate-" Godtfredsen et al. Proceedings of the European Brewery Convention, 1986 pp. 161-168
Carlsberg Res. Commun., vol. 48, 1983, pp. 239-247; S. E. Godtfredsen: "On the Occurrence of Alpha-Acetolactate Decarboxylases Among Microorganisms".
EBC Congress 1983, vol. 89, lecture No. 17, pp. 161-168; S. E. Godtfredsen et al.: "Use of Alpha-Acetolactate Decarboxylase for Accelerated Maturation of Beer".

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