Purification of Il-2-containing hybrid compounds

Details Purification of Il-2-containing hybrid compounds Purification of Il-2-containing hybrid compounds

1990-03-02

1992-05-05

Draper, Garnette D.

Chemistry: natural resins or derivatives; peptides or proteins;

Proteins, i.e., more than 100 amino acid residues

Separation or purification

530351, 530412, 530417, 530402, 530422, 424 852, C07K 320

Patent

active

051109123

ABSTRACT:
A method for purifying from a mixture of hybrid compound which contains a portion of IL-2, which portion includes at least a region of the IL-2R binding domain of IL-2, which region is effective to cause the hybrid compound to bind to cells containing an IL-2R. The method includes passing the mixture containing the hybrid compound over a column having attached thereto molecules consisting of or containing a complex sugar moiety with affinity for the hybrid compound, and eluting the hybrid compound with a suitable eluant. The invention also features related complexes of the hybrid compound and assays for the hybrid compound.

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patent: 4675382 (1987-06-01), Murphy
patent: 4771128 (1989-09-01), Ferris et al.
patent: 4892827 (1990-01-01), Pastan et al.
Lorberbourn-Galski et al, PNAS 85, 1984, pp. 1922-1926.
Kelley, PNAS 1988, vol. 85, pp. 3980-3984.
Bailon et al, Biotechnolog, Nov. 1988, pp. 1326-1329.
Perendesis et al PNAS 85, 1988, pp. 8386-8390.
Chaudhary et al Nature 339, 1989, pp. 394-397.
Kirkman et al, Transplantation 47, 1989, pp. 327-330.
Williams et al, Protein Engineering vol. 1(6) 1987, pp. 493-498.
Lorberbourn-Galski et al, IBC 263(35) 1988, pp. 18650-18656.

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