Process for purification of crude kallikrein

Chemistry: molecular biology and microbiology – Enzyme – proenzyme; compositions thereof; process for... – Pancreatin

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435188, 435213, 435226, 435815, C12N 950, C12N 994

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active

042529026

ABSTRACT:
A process for purification of crude kallikrein utilizing a specific affinity of kallikrein to p-aminobenzamidine, which comprises bringing a solution, containing kallikrein and contaminated by other undesirable enzymes and proteins, into contact with a water-insoluble carrier combined with p-aminobenzamidine, exclusively eluting out the other enzymes and proteins from the carrier by the use of a buffer solution of lower NaCl concentration and then exclusively eluting out kallikrein by the use of a buffer solution of higher NaCl concentration.

REFERENCES:
patent: 3100736 (1963-08-01), Werle et al.
patent: 3746622 (1973-07-01), Nishikawa et al.
patent: 3912595 (1975-10-01), Philipp et al.
patent: 3994782 (1976-11-01), Tokuyasu et al.
patent: 4020268 (1977-04-01), Nishikawa et al.
patent: 4064010 (1977-12-01), Harris et al.

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