Process for recovering microbial cellular proteins

Chemistry of carbon compounds – Miscellaneous organic carbon compounds – C-metal

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A23J 118

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040726673

ABSTRACT:
Proteins contained in microbial cells, for example, cells of petroleum-assimilable yeast, are separated and purified by extracting proteins from microbial cells, treating the resulting extract solution with a cation exchanger, treating said cation exchanger containing the adsorbed proteins with a volatile basic substance in the presence of water thereby to desorb or elute the adsorbed proteins, removing said basic substance dissolved in the resulting eluate by evaporation, and then concentrating the eluate thereby to obtain the dissolved proteins.

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Methods in Enzymology, vol. XXII, 1971, Jakoby, pp. 273-286.
Biochemical J., vol. 59, 1955, pp. 543-552, Boardman et al.
Kunin, "Ion Exchange Resins", 2nd ed., John Wiley & Sons, New York, p. 92.
Calmon and Kressman, "Ion Exchangers in Organic and Biochemistry", Interscience Pub., New York, 1957, p. 124.

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