Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Recombinant dna technique included in method of making a...
Patent
1987-06-03
1990-07-31
Teskin, Robin
Chemistry: molecular biology and microbiology
Micro-organism, tissue cell culture or enzyme using process...
Recombinant dna technique included in method of making a...
435 691, 4351723, 435320, 435255, 435256, 935 37, 935 28, 935 69, 536 27, C12P 2100, C12N 1500, C12N 500
Patent
active
049450468
ABSTRACT:
A method of producing a heterologous protein in yeast is disclosed, which comprises transforming a yeast Saccharomyces cerevisiae with recombinant DNA comprising a promoter selected from the group consisting of a yeast promoter or a hybrid promoter derived from a yeast promoter, particularly a hybrid promoter containing the enhancer region of SV40 virus, and a gene coding for a heterologous protein such as HBsAg and Pre S-HBsAg, particularly full length Pre S-HBsAg culturing, the resulting transformed cells to express the gene coding the heterologous protein, and isolating the heterologous protein from the cultured medium. GAP-DH and PH05 promoters and miniaturized ones can be used as the yeast promoter. The expression of the full length Pre S-HBsAg is carried out in the same expression system as that for 2nd or 3rd Pre S-ABsAg.
REFERENCES:
Bitter et al., Gene vol. 32, 1984, pp. 263-274 "Expression of Heterologous Genes in Saccharomyces cerevisiae from Vectors Utilizing the Glyceraldehyde-3-Phosphate Dehydrogenase Gene Promoter".
Khoury et al., Cell, vol. 33, pp. 313-314, Jun. 1983, "Enhancer Elements".
Valenzuela et al., Biotechnology, vol. 3, p. 317, 1985.
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Jones et al., Chem. Abst., vol. 104 (7), No. 49438g, 1988.
Gelfane et al., Chem. Abst., vol. 104(25), No. 220131z, 1985 "Mammalian Promoters Useful in Yeast Expression".
Guarente et al., Proc Natl Acad. Sci., vol. 79, Dec. 1982, pp. 7410-7414, "A Gal10-Cyc1 Hybrid Yeast Promotor Identifies the Gal4 Regulatory Region".
Murray et al., EMBC, vol. 3(3), pp. 645-650, 1984, "Hepatitis B Virus Antigens Made in Microbial cells Immunis Against Viral Infection".
Valenzuela et al., "Synthesis and Assembly in Yeast . . . Receptor", 6062 Biotechnology, 3 (1985) Apr., No. 4, London, Great Britain, pp. 317-320.
K. Struhl, "Genetic Properties and Chromatin . . . Sequence", Proc. Natl. Acad. Sci. U.S.A., vol. 81, pp. 7865-7869, Dec. 1984.
Arimura Hirofumi
Horii Hajime
Kawabe Haruhide
Kobayashi Kaoru
Mukai Hiromichi
Teskin Robin
The Green Cross Corporation
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