Processes for purifying synthetic oligonucleotides

Details Processes for purifying synthetic oligonucleotides Processes for purifying synthetic oligonucleotides

1997-03-12

1998-06-23

Houtteman, Scott W.

Organic compounds -- part of the class 532-570 series

Organic compounds

Carbohydrates or derivatives

536 2541, C07H 2100

Patent

active

057707235

ABSTRACT:
Purification of synthesized oligonucleotides is achieved using novel processes and reagents. The novel processes involve adding certain salts to treated synthesized oligonucleotides prior to eluting the oligonucleotides from reverse phase purification cartridges. The novel processes also involve treating synthesized oligonucleotides using cleaving and deprotecting reagents containing methylamine and certain salts. Reagents comprising methylamine and certain salts can be used to rapidly cleave and deprotect oligonucleotides while obtaining high yields of purified oligonucleotides using reverse phase purification technologies.

REFERENCES:
"Fast Cleavage and Deprotection of Oligonucleotides", M.P. Reddy et al., Tetrahedron Letters, vol. 35, No. 25, pp. 4311-4314, 1994 (Elsevier Science Ltd., Printed in Great Britain).
"A New, Reliable Cartridge for the Rapid Purification of Synthetic DNA", L.J. McBride et al., 362 BioTechniques, vol. 6, No. 4 (1988).

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