Discriminatory substrates for MMP hydrolysis

Details Discriminatory substrates for MMP hydrolysis Discriminatory substrates for MMP hydrolysis

1995-06-05

1998-06-23

Leary, Louise

Chemistry: natural resins or derivatives; peptides or proteins;

Peptides of 3 to 100 amino acid residues

8 to 10 amino acid residues in defined sequence

530300, 435 23, 435 24, 424 49, 424 943, A61K 3804, A61K 3800, A61K 716, C12Q 137

Patent

active

057706913

ABSTRACT:
A method of screening a sample for the presence of a matrix metalloproteinase employing discriminatory peptide substrates is provided. The method involves providing a peptide substrate of 6-14 amino acid residues containing at least one matrix metalloproteinase cleavage site. The peptide substrate contains Mca as a fluorogenic group and Lys(Dnp) as a quenching group separated by at least four amino acid residues, wherein the peptide substrate is specific for the matrix metalloproteinase of interest. The peptide substrate is combined with a sample containing at least one matrix metalloproteinase to form a mixture. The fluorescence of the mixture is monitored to determine if the matrix metalloproteinase of interest is present.

REFERENCES:
Weingarten et al., "Synthetic Substrates of Vertebrate Collagenase," Biochemistry, 24, 6730-6734 (1985). Month not available.
Wilhelm et al., "Matrix Metalloproteinase-3 (Stromelysin-1); Identification as the cartilage acid metalloprotease and effect of pH on catalyic properties and calcium affinity," J. Biol. Chem., 268, 21906-21913 (Oct. 15, 1993).
Wu et al., "Sites of Stromelysin Cleavage in Collagen Types II, IX, X, and XI of Cartilage," J. Biol. Chem., 266, 5625-5628 (Mar. 25, 1991).
Ogata et al., "Matrix Metalloproteinase 3 (Stromelysin) Activates the Precursor for the Human Matrix Metalloproteinase 9," J. Biol. Chem., 267, 3581-3584 (Feb. 25, 1992).
Okada et al., "Immunolocalisation of matrix metalloproteinase 3 (stromelysin) in rheumatoid synovioblasts (B cells): correlation with rheumatoid arthritis," Annals of the Rheumatic Diseases, 48, 645-653 (Aug. 1989).
Okada et al., "Matrix metalloproteinase 2 from human rheumatoid synovial fibroblasts; Purification and activation of the precursor and enzymic properties," Eur. J. Biochem., 194, 721-730 (Dec. 27, 1990).
Poe et al., "High Pressure Gel-Permeation Assay for the Proteolysis of Human Aggrecan by Human Stromelysin-1: Kinetic Constants for Aggrecan Hydrolysis," Arch. Biochem. Biophys., 298, 757-759 (Nov. 1, 1992).
Stack et al., "Comparison of Vertebrate Collagenase and Gelatinase Using a New Fluorogenic Substrate Peptide," J. Biol. Chem., 264, 4277-4281 (Mar. 15, 1989).
Stetler-Stevenson et al., "Tumor cell interactions with the extracellular matrix during invasion and metastasis," Annu. Rev. Cell. Biol., 9, 541-573 (1993). Month not available.
Suzuki et al., "Mechanisms of Activation of Tissue Procollagenase by Matrix Metalloproteinase 3 (Stromelysin)," Biochemistry, 29, 10261-10270 (1990). Month not available.
Teahan et al., "Substrate Specificity of Human Fibroblast Stromelysin. Hydrolysis of Substance P and its Analogues," Biochemistry, 28, 8497-8501 (1989). Month not available.
Weingarten et al., "Cleavage site specificity of vertebrate collagenases," Biochem. Biophys. Res. Commun., 139, 1184-1187 (Sep. 30, 1986).
Mast et al., "Kinetics and Physiologic Relevance of the Inactivation of .alpha..sub.1 -Proteinase Inhibitor,.alpha..sub.1 -Antichymotrypsin, and Antithrombin III by Matrix Metalloproteinases-1 (Tissue Collagenase), -2 (72-kDa Gelatinase/Type IV Collagenase), and -3 (Stromelysin)," J. Biol. Chem., 266, 15810-15816 (Aug. 25, 1991).
Morodomi et al., "Purification and characterization of matrix metalloproteinase 9 from U937 monocytic leukaemia and HT1080 fibrosarcoma cells," Biochem. J., 285, 603-611 (Jul. 15, 1992).
Nagase et al., "Design and Characterization of a Fluorogenic Substrate Selectively Hydrolyzed by Stromelysin 1 (Matrix Metalloproteinase-3)," J. Biol. Chem., 269, 20952-20957 (Aug. 19, 1994).
Nagase et al., "Stepwise Activation Mechanisms of the Precursor of Matrix Metalloproteinase 3 (Stromelysin) by Proteinases and (4-Aminophenyl)mercuric Acetate," Biochemistry, 29, 5783-5789 (1990). Month not available.
Netzel-Arnett et al., "Comparative Sequence Specifities of Human 72- and 92-kDa Gelatinases (Type IV Collagenases) and PUMP (Matrilysin)," Biochemistry, 32, 6427-6432 (1993). Month not available.
Netzel-Arnett et al., "Continuously Recording Fluorescent Assays Optimized for Five Human Matrix Metalloproteinases," Anal. Biochem., 195, 86-92 (1991). Month not available.
Netzel-Arnett et al., "Sequence Specifities of Human Fibroblast and Neutrophil Collagenases," J. Biol. Chem, 266, 6747-6755 (Apr. 15, 1991).
Niedzwiecki et al., "Substrate Specificity of the Human Matrix Metalloproteinase Stromelysin and the Development of Continuous Fluorometric Assays," Biochemistry, 31, 12618-12623 (1992). Month not available.
Fields, G.B. et al., "Principles and Practice of Solid-Phase Peptide Synthesis," Synthetic Peptides: A User's Guide, G.A. Grants, Ed.; W.H. Freeman: New York; pp. 77-183 (1992).
Fields, G.B. et al., "Sequence Specificity of Human Skin Fibroblast Collagenase; Evidence for the Role of Collagen Structure in Determining the Collagenase Cleavage Site," J. Biol. Chem., 262, 6221-6226 (May 5, 1987).
Flannery et al., "Identification of a Stromelysin Cleavage Site within the Interglobular Domain of Human Aggrecan," J. Biol. Chem., 267, 1008-1014 (Jan. 15, 1992).
Geoghegan et al., "Site-Directed Double Fluorescent Tagging of Human Renin and Collagenase (MMP-1) Substrate Peptides Using the Periodate Oxidation of N-Terminal Serine. An Apparently General Strategy for Provision of Energy-Transfer Substrates for Proteases," Bioconjugate Chem., 4, 537-544 (1993). Month not available.
Ito et al., "Evidence that Human Rheumatoid Synovial Matrix Metalloproteinase 3 is an Endogenous Activator of Procollagenase," Archives of Biochem. Biophys., 267, 211-216 (Nov. 15, 1988).
King et al., "A cleavage method which minimizes side reactions following Fmoc solid phase peptide synthesis," Int. J. Peptide Protein Res., 36, 255-266 (Sep. 1990).
Knauper et al., "Direct activation of human neutrophil procollagenase by recombinant stromelysin," Biochem. J., 295, 581-586 (Oct. 15, 1993).
Knight et al., "A novel coumarin-labelled peptide for sensitive continuous assays of the matrix metalloproteinases," FEBS Lett., 296, 263-266 (Jan. 1992).
Azzo et al., "Purification and Characterization of an Acid Metalloprotease from Human Articular Cartilage," J. Biol. Chem., 261, 5434-5441 (Apr. 25, 1986).
Berman et al., "Rapid Optimization of Enzyme Substrates Using Defined Substrate Mixtures," J. Biol. Chem., 267, 1434-1437 (Jan. 25, 1992).
Bickett et al., "A High Throughput Fluorogenic Substrate for Interstitial Collagenase (MMP-1) and Gelatinase (MMP-9)," Anal. Biochem., 212, 58-64 (1993). Month not available.
Enghild et al., "Interaction of Human Rheumatoid Synovial Collagenase (Matrix Metalloproteinase 1) and Stromelysin (Matrix Metalloproteinase 3) with Human .alpha..sub.2 -Macroglobulin and Chicken Ovostatin," J. Biol. Chem., 264, 8779-8785 (May 25, 1989).
Fields, C.G. et al., "The Development of High-Performance Liquid Chromatographic Analysis of Allyl and Allyloxycarbonyl Side-Chain-Protected Phenylthiohydantoin Amino Acids," Anal. Biochem., 203, 245-251 (1992). Month not available.
Fields, C.G. et al., "Edman Degradation Sequence Analysis of Resin-Bound Peptides Synthesized by 9-Fluorenylmethoxycarbonyl Chemistry," Peptide Res., 6, 39-46 (1993). Month not available.
Fields, C.G. et al., "HBTU Activation for Automated Fmoc Solid-Phase Peptide Synthesis," Peptide Res., 4, 95-101 (1991). Month not available.
Fields, G.B., "The Application of Solid Phase Peptide Synthesis to the Study of Structure Function Relationships in the Collagen-Collagenase System," Ph.D. Thesis; Florida State University, Tallahassee, FL; pp. 136-176 (1988). Month not available.
Fields, G.B., "A Model for Interstitial Collagen by Mammalian Collegenases," J. theor. Biol., 153, 585-602 (1991). Month not available.
C. G. Fields et al., "Edman degradation sequence analysis of resin-bound peptides: Characterization of unusual and side chain protected PTH-amino acids," in Peptides 1992; C. H. Schneider et al., Eds.; ESCOM: Leiden, The Netherlands; pp. 447-448 (1992). Month not available.
Nagase et al., "Substrate specificities and activation mechanisms of matrix metalloproteinases," Biochem. Soc. Trans. (U.K.), 19(3), 715-718 (1

Affiliated with

Also associated with

Rating

Discriminatory substrates for MMP hydrolysis does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Discriminatory substrates for MMP hydrolysis, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Discriminatory substrates for MMP hydrolysis will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-1395438