Method for the determinate evaluation of a chemistry analyzer's

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364554, 73 1H, G06F 1520

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active

051464136

ABSTRACT:
A method for chemistry systems analysis to identify the performance of the functional components of the system, specifically, those of volumes dispensed and optical linearity. Employing a linear regression comparison between predetermined absorbance values, calculated from Beer's Law, and absorbance values obtained from the optical system under test, a value correction constant derived from the regression comparison may be applied to all subsequent absorbance values. Absorbance values, obtained from the optical system as a result of analyzing dilutions are by the pipetting system of material having a greater concentration but the same molar absorbtivity constant as the material used for the linearity evaluation, can be corrected to a value that does not reflect any optical system bias, and may be considered as the divident for the function of converting absorbance values into volumes dispensed. The divisor of the convertion function will be derived from the predetermined absorbance of the concentrate, using Beer's Law, and dividing that by the total volume requested by the volumes evaluation, leaving an absorbance value that equals a lul delivery. The volume dispensed by the pipetting system may then be calculated by dividing the corrected absorbance value by the lul absorbance value, yielding a quotient identifying the actual volume delivered.

REFERENCES:
patent: 4354376 (1988-10-01), Greenfield et al.
Lambbeck; "Calibration of Small Volumetric Laboratory Glassware"; Institute For Basic Standards Dec. 19, 1974.

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