Modification of cryptic splice sites in heterologous genes expre

Details Modification of cryptic splice sites in heterologous genes expre Modification of cryptic splice sites in heterologous genes expre

1996-06-27

1998-06-23

Guzo, David

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

435 914, 43525411, 4353201, 435 691, 536 231, C12Q 168, C12P 1934, C12N 115, C07H 2104

Patent

active

057703710

ABSTRACT:
The present invention relates to methods for obtaining a fungal host cell comprising a nucleic acid sequence encoding a heterologous polypeptide, wherein at least one cryptic splice site is modified in the nucleic acid sequence. The present invention also relates to a nucleic acid sequence(s) with a modified cryptic splice site(s) as well as nucleic acid constructs, vectors, and host cells comprising said nucleic acid sequence(s). The present invention further relates to methods for recombinant production of a polypeptide encoded by said nucleic acid sequence.

REFERENCES:
patent: 5498531 (1996-03-01), Jarrell
Chanfreau et al.. Interaction between the first and last nucleotides of pre-mRNA introns is a determinant of 3' splice site selection in S. cerevisiae. Nuc. Acids Res. vol. 22(11):1981-1987, Jun. 11, 1994.
Guo et al.. The mobile group 1 intron 3 alpha of the yeast mitochondrial COXI gene encodes a 35-kDA processed protein that is an endonuclease but not a maturase. J. Biol. Chem. vol. 270(26):15563-15570, Jul. 11, 1995.

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