Process for the activating of gene-technologically produced, het

Details Process for the activating of gene-technologically produced, het Process for the activating of gene-technologically produced, het

1995-06-01

1997-01-14

Stanton, Brian R.

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Recombinant dna technique included in method of making a...

435 711, 435 712, 435849, 435877, 530402, 530404, 530412, 530414, C12P 104, C12P 2102, C12N 1500, C12N 1563

Patent

active

055938655

ABSTRACT:
A process for the activation of disulphide linked recombinant proteins expressed in prokaryotes is described. The process includes cell digestion, solubilization under denaturing and reducing conditions and activation under oxidizing conditions in the presence of GSH/GSSG and a non-denaturing amount of a denaturing agent.

REFERENCES:
patent: 4432895 (1984-02-01), Tarnowski
patent: 4511502 (1985-04-01), Builder et al.
patent: 4512922 (1985-04-01), Jones et al.
patent: 4599197 (1986-07-01), Wetzel
patent: 4705848 (1987-11-01), Yang et al.
patent: 4766075 (1988-08-01), Goeddel et al.
patent: 5077392 (1991-12-01), Rudolph et al.
patent: 5453363 (1995-09-01), Rudolph et al.

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