Process for the production of protease inhibitors

Details Process for the production of protease inhibitors Process for the production of protease inhibitors

1996-09-16

1998-03-10

Elliott, George G.

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Recombinant dna technique included in method of making a...

435 712, 4352551, 4353201, C12P 2106, C12N 115, C12N 1581

Patent

active

057260435

ABSTRACT:
A novel process for the production of recombinant desulphatohirudin by transformed yeast strains is provided. The process makes use of an expression cassette comprising the yeast CUP1 promoter. The invention concerns also said transformed yeast strains, novel expression vectors and methods for the production thereof.

REFERENCES:
patent: 4940661 (1990-07-01), Etcheverry et al.
patent: 5162208 (1992-11-01), Lemoine et al.
Bailey et al, "Biochemical Engineering Fundamentals", 2nd Ed. (1986): pp. 384-384.
Henderson et al, "The Transformation of Brewing Yeasts with a Plasmid Containing the Gene for Copper Resistance" Current Genetics (1985): pp. 133-138.
Bergmeyer, M.U. (ed.), Methods in Enzymatic Analysis, vol. II, pp. 314-316 (1983).
Butt et al., Microbiol. Rev., "Yeast metallothionein and applications in biotechnology," 51:pp. 351-364 (1987).
Butt et al., PNAS, USA, "Copper metallothionein of yeast, structure of the gene, and regulation of expression," 81: pp. 3332-3336 (1984).
Dohmen et al., Curr. Genet., "Regulated overproduction of a-amylase by transformation of the amylolytic yeast Schwanniomyces occidentalis," Curr. Genet., 15:pp. 319-325.
Etcheverry, T., Methods Enzymol., "Induced expression using yeast copper metallothionein promoter," 185:pp. 319-329 (1990).
Furst et al., Cell, "Copper activates metallothionein gene transcription by altering the conformation of a specific DNA binding protein," 55:pp. 705-717 (1988).
Hamer et al., Science, "Function and autoregulation of yeast copperthionein," 228:pp. 685-690 (1985).
Hanahan, D., J. Mol. Biol., "Studies on transformation of Escherichia coli with plasmids," 166:pp. 557-580 (1983).
Herskowitz and Jensen, Methods in Enzymology, "Putting the HO gene to work:practical uses for mating-type switching," 194:pp. 132-146 (1991).
Huibregtse et al., PNAS USA, "Copper-induced binding of cellular factors to yeast metallothionein upstream activation sequences," 86:pp. 65-69 (1989).
Markwardt et al., Thromb. Haemost., "Pharmacological studies on the antithrombotic action of hirudin in experimental animals," 47(3):pp. 226-229 (1982).
Rudolph et al., Gene, "One-step gene replacement in yeast by cotransformation," 36:pp. 87-95 (1982).
Scharf et al., FEBS Lett., "Primary structures of new `iso-hirudins`", 255:pp. 105-110 (1989).
Silar et al., Mol. Cell. Biol., "Heat shock transcription factor activates transcription of the yeast metallothionein gene," 11(3):pp. 1232-1238 (1991).
Sprague, G.F., Methods in Enzymology, "Assay of yeast mating reaction," 194:pp. 77-93 (1991).
Thiele et al., Science, "Mammalian metallothionein is functional in yeast," 231:pp. 854-856 (1986).
Welch et al., EMBO J., "The CUP2 gene product regulates the expression of the CUP1 gene, coding for yeast metallothionein," 8:pp. 255-260 (1989).
Wright et al., Nucleic Acids Res., "Chromogenic identification of oligonucleotide-directed mutants," 14:pp. 8489-8499 (1986).
Zoller and Smith, Methods in Enzymology, "Oligonucleotide-directed mutagenesis of DNA fragments cloned into M13 vectors," 100:pp. 468-500 (1983).
Sherman, F., Methods in Enzymology, "Getting Started with Yeast", 194:pp. 3-21 (1991).
Moir, D.T. et al. (1991) in Methods in Enzymology v. 194, Eds. Guthrie et al, pp. 491-507 Academic.
White way et al. (1984) Molec. Cell, Biol. Press. NY 4/(1), pp. 195-198.
Macreadie, IG et al. (1991). Gene 104,pp. 107-111.
Ordman A.B. et al. (1991), J. Steroid Biochem. Molec. Biol., 39 (4A), pp. 487-492.
Scott, J.F. et al., (1987), in Biological Research on Industrial Yeasts, Eds. Stewart et al. CRC Press Boca Raton, Fla. pp. 38-41.
Takabayashi, K. et al (1990) Yeast 6, p. 11-6A (Abstract).

Affiliated with

Also associated with

Rating

Process for the production of protease inhibitors does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Process for the production of protease inhibitors, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Process for the production of protease inhibitors will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-138523