Method for integrative transformation of yeast using dispersed r

Chemistry: molecular biology and microbiology – Vector – per se

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435 6, 435 696, 4351723, 536 241, 536 242, 935 28, 935 55, 935 68, 935 69, C12N 1500, C12N 1509, C12N 1563, C12N 1581

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056292031

ABSTRACT:
Efficient integration of heterologous DNA into yeast genomic DNA is accomplished at high copy number by targeting integration vectors to dispersed repetitive elements such as DELTA sequences, Ty elements, or tRNA DNA sequences present in the host cell genome.

REFERENCES:
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Futcher et al., (1984 Journal of Bacteriology 157:283-290.
Karin et al., (1984) Proc. Natl. Acad. Sci. 81: 337-341.
Lopes et al., (1989) Gene 79:199-206.
Orr-Weaver et al., (1983) Methods in Enzymology 101:228-245.
Smith et al., (1985) Science 229:1219-1224.
Warmington et al., (1985) Nucleic Acids Research 13(18):6679-6693.
Cousins et al, Gene 61:265-275 (1987)"High Level Expression of Proinsulin in yeast, S. cerevesiae".
Warmington et al, Nucleic Acids Research 13:6679 Nucleotide Sequence Characterization of Tyl-17, a Class II Transposon.
Lopes et al, Gene 79: 199-206 (1989) High Copy Number Integration into the Vibosomal DNA of S. cerevesiae.
Karin, PNAS 81:337-341 (1984) Primary Structure . . . CUP 1 Locus.

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