Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving viable micro-organism
Patent
1995-09-11
1997-05-13
Kight, John
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving viable micro-organism
435 29, 435 4, 435262, 435170, 435 41, C12Q 104, C12Q 102, C12Q 100, C12P 100
Patent
active
056291701
ABSTRACT:
An assay method for screening microorganism-containing compositions for degradation capacity via the 2,3-dioxygenase pathway includes a first step of contacting the microorganism with a polychlorinated aromatic compound which is not susceptible to such degradation but which, when anaerobically dechlorinated, affords products which are susceptible to such degradation. The resulting product is contacted in a second step with an indicator microorganism encoding the first three steps but not the fourth step of the 2,3-dioxygenase route, and the presence of color in the resulting product is an indication that dechlorination took place in the first step. The presence in a test sample of halogenated aromatic compounds capable of degradation by the 2,3-dioxidase pathway can be determined by employing the second step alone.
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General Electric Company
Kight John
Leary Louise
Pittman William H.
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