Solid phase diagnosis of medical conditions

Details Solid phase diagnosis of medical conditions Solid phase diagnosis of medical conditions

1995-06-07

1997-05-13

Zitomer, Stephanie W.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

435 912, 935 77, 935 78, C12P 1934, C12Q 168

Patent

active

056291582

ABSTRACT:
A method of and kit for diagnosis of medical conditions by identification of specific DNA characterized in that DNA in a sample is subjected to initial amplification by the polymerase chain reaction (PCR) method using a first pair of primers specific to the target DNA and the amplified DNA so produced is further amplified by the PCR method using a second primer pair, one or both of which are different from either of said first primer pair and are specific to a sequence or sequences of the target DNA between the hybridization sites of said first primer pair, one of the primers of said second primer pair being immobilized on a solid support or being provided with means for subsequent attachment to a solid support and the other of said second pair of primers carrying a label or being provided with means for subsequent attachment to a label, said amplification being followed by separation of said solid support carrying said amplified DNA and detection of label attached thereto, one or both of said means for subsequent attachment comprising a distal DNA sequence carried by the said primer which does not hybridize with the target DNA but has a selective affinity for a binding partner attached to either a solid support or a label.

REFERENCES:
patent: 4777129 (1988-10-01), Dattagupta et al.
patent: 4965188 (1990-10-01), Mullis et al.
Mullis et al., Cold Spring Harb. Symp. Quant. Biol. 51:263-273(1986).
Venetianer et al., PNAS, USA 71(10):3892-3895 (Oct. 1974).
Syvanen et al., Nuc. Acids Res. 16(23):11327-11338.
Kemp et al. (Apr. 1989) Proc. Nat'l Acad. Aci., U.S.A.86:2423 "Colorimetric detection of specific DNA polymerase chain reactions".

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