Inhibition of II synthesis

Details Inhibition of II synthesis Inhibition of II synthesis

1996-06-11

1998-03-10

Elliott, George C.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

435 72, 435 721, 4351721, 4353201, 435326, 536 245, 536 241, C12Q 168, G01N 3353, C07H 2100, C12N 500

Patent

active

057260206

ABSTRACT:
Disclosed are expressible reverse gene constructs, and oligonucleotides, which are characterized by the ability to hybridize with an Ii mRNA molecule, thereby inhibiting translation of the Ii mRNA molecule. These compositions are referred to generally as inhibitors of Ii expression. Also disclosed are MHC class II-positive antigen presenting cells containing an inhibitor of Ii expression. A particularly important class of MHC class II-positive antigen presenting cells are malignant MHC class II-positive antigen presenting cells (e.g., leukemia, lymphoma and melanoma). Also disclosed are methods which results in the display of an autodeterminant peptide, in association with an MHC class II protein, on the surface of an MHC class II-positive antigen presenting cell. In such methods, a specific inhibitor of Ii synthesis is introduced into an MHC class II-positive antigen presenting cell. The specific inhibitor functions, directly or indirectly, through the formation of a duplex molecule with mRNA encoding Ii. The formation of the duplex molecule functions to inhibit Ii synthesis at the translational level. Also disclosed are therapeutic methods for the treatment of malignancy in an MHC class II-positive antigen presenting cell, and autodeterminant peptides which are isolated from an MHC class II-positive antigen presenting cell.

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