Measuring composition

Details Measuring composition Measuring composition

1995-06-06

1996-04-09

Gitomer, Ralph J.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving glucose or galactose

435 15, 435 17, 435 26, C12Q 154, C12Q 132

Patent

active

055061133

ABSTRACT:
A measuring composition for use in determination of glucose, ATP, phosphotransferases such as creatine phosphokinase, and glucosidases is disclosed. The composition is comprised of glucokinase, glucose-6-phosphate dehydrogenase, and potassium or ammonium ions. This composition makes it possible to shorten measuring times even if the amount of enzyme is reduced, and exhibits high stability when stored at room temperature in a solution state.

REFERENCES:
patent: 4271264 (1981-06-01), Modrovich
patent: 4438199 (1984-03-01), Miwa et al.
Walker et al (1966) Glucokinase "Methods in Enzgmology" 9:381-8 Academic Press.
Kruse et al, Tissue Culture Methods and Applications, Academic Press, New York, 685 (1973).
Walker et al, "Glucokinase", Methods Of Enzymology, vol. 9, pp. 381-388 (1966) Academic Press.
Kamel et al, "An Ultraspecific Micromethod for Determination of Deglycose", Analytical Biochemistry, 18, 270-273 (1967).

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