Process for producing aspartase and process for producing L-aspa

Details Process for producing aspartase and process for producing L-aspa Process for producing aspartase and process for producing L-aspa

1997-03-24

1999-06-29

Weber, Jon P.

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Preparing alpha or beta amino acid or substituted amino acid...

435232, 435 711, 435 712, C12P 1320, C12P 2100, C12N 988

Patent

active

059167823

ABSTRACT:
Disclosed are a process for producing aspartase which comprises culturing a microorganism belonging to the genus Escherichia being capable of producing aspartase in a medium until aspartase is produced and accumulated in the culture, and recovering aspartase therefrom, wherein dissolved oxygen concentration of the medium is in the range of 0 to 1 ppm on the stage that microbial growth is middle and/or late logarithmic growth phase; and a process for producing L-aspartic acid which comprises converting fumaric acid and ammonia into L-aspartic acid in an aqueous medium in the presence of an enzyme source, wherein said enzyme source is a culture produced in accordance with said aspartase-producing process, cells isolated from the culture, or processed cells thereof.

REFERENCES:
patent: 4436813 (1984-03-01), Wood et al.
patent: 4560653 (1985-12-01), Sherwin et al.
patent: 4698224 (1987-10-01), Nakanishi et al.
patent: 5441634 (1995-08-01), Edwards et al.
patent: 5541090 (1996-07-01), Sakano et al.
"Reaction Process. A New Production Process of L-Asparatic Acid" Masato, et al. (1994) vol. 58 No. 11 pp. 878-882, (with English abstract).
Smith et al. (1984) Eur. Congr. Biotechnol. 3 Meet, 1, "Biosynthesis of Aspartase and Production of Aspartic Acid ina Hollow Membrane Fiber Immobilized-Cell Reactor", p. 475, in Biotechds, AN 85-11128.

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