Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Preparing compound containing saccharide radical
Patent
1997-06-18
1999-06-29
Degen, Nancy
Chemistry: molecular biology and microbiology
Micro-organism, tissue cell culture or enzyme using process...
Preparing compound containing saccharide radical
4351737, 435182, 536 254, C12P 1934, C12N 1300, C12N 1100, C07H 2104
Patent
active
059167750
ABSTRACT:
Disclosed is a method for collecting DNA by lysing microbial cells, adsorbing released DNA on a carrier and collecting the DNA adsorbed on the carrier, which method comprises the following steps of (1) lysing the microbial cells in the presence of the carrier so that the DNA obtained by lysing cells is adsorbed onto the carrier, separating solutions used for lysing cells and adsorbing DNA from the carrier, and eluting the DNA adsorbed on the carrier with a solution for eluting DNA and collecting eluted DNA, or (2) feeding microbial cells into a column comprising the carrier provided on a membrane filter capable of retaining a solution and permeating the solution when aspirated, lysing the microbial cells in the column so that the DNA obtained by lysing cells is adsorbed onto the carrier, separating solutions used for lysing cells and adsorbing DNA in the previous step from the column by aspiration, and feeding a solution for eluting DNA into the column and aspirating to collect the DNA adsorbed on the carrier. The methods of the present invention enable collection of DNA by the chaotropic ion method employing an apparatus with simpler structure and fewer operations.
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Degen Nancy
Sandals William
The Institute of Physical and Chemical Research
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