Trehalose-releasing enzyme

Details Trehalose-releasing enzyme Trehalose-releasing enzyme

1994-06-02

1995-12-05

Naff, David M.

Chemistry: molecular biology and microbiology

Enzyme , proenzyme; compositions thereof; process for...

Hydrolase

435201, 435195, 435193, 435100, 53612313, C12N 924, C12N 926, C12N 914, C12N 910

Patent

active

054728630

ABSTRACT:
A trehalose-releasing enzyme is obtained which specifically hydrolyzes the linkage between a trehalose moiety and the remaining glycosyl moiety in a non-reducing saccharide having a trehalose structure as an end unit and having a degree of glucose polymerization of 3 or higher. The molecular weight of the enzyme is about 57,000 to 68,000 daltons on SDS-PAGE, and the isoelectric point is about 3.3 to 4.6 on isoelectrophoresis. The enzyme is preferably obtained from Rhizobium sp. FERM BP-4130 or Arthrobacter sp. FERM BP-4316, and may also be obtained from members of the genera Brevibacterium and Micrococcus. The enzyme is useful in an industrial-scale preparation of trehalose, and the trehalose prepared therewith can be readily incorporated into food products, as well as cosmetic- and pharmaceutical-compositions.

REFERENCES:
patent: 4762857 (1988-08-01), Bollin, Jr. et al.
patent: 5169767 (1992-12-01), Matsuura et al.
patent: 5218096 (1993-06-01), Shibuya et al.

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