DNA encoding lemA-independent GacA and its use in activating gen

Details DNA encoding lemA-independent GacA and its use in activating gen DNA encoding lemA-independent GacA and its use in activating gen

1998-01-20

1999-04-06

Railey, II, Johnny F.

Chemistry: molecular biology and microbiology

Treatment of micro-organisms or enzymes with electrical or...

Modification of viruses

4352523, 43525234, 4353201, 530350, 536 237, C07K 1421, C12N 121, C12N 1531, C12N 1563

Patent

active

058916881

ABSTRACT:
The present invention exploits the discovery that certain single base changes in the gacA gene coding sequence, which result in certain single amino acid changes in the encoded GacA protein, render it LemA-independent. The present invention therefore provides DNA that encodes mutant GacA proteins that do not require phosphorylation by LemA in order to be active as transcriptional activators.

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Hrabak et al., "The lemA Gene Required for Pathogenicity of Pseudomonas syringae pv. Syringae on Bean Is a Member of a Family of Two-Component Regulators", J. of Bacteriology, 174(9): 3011-3020 (1992).
Laville et al., "Global control in Pseudomnas fluroescens mediating antibiotic synthesis and suppression of black root rot of tabacco", Proc. Natl. Acad. Sci., 39:1562-1566 (1992).
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