Method and apparatus for determining the role of cytochrome P450

Details Method and apparatus for determining the role of cytochrome P450 Method and apparatus for determining the role of cytochrome P450

1993-09-27

1995-12-26

Wityshyn, Michael G.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

435 25, 435189, 435966, 436 63, 436 64, 530846, C12Q 100, C12Q 126, C12N 902, G01N 33483

Patent

active

054787238

ABSTRACT:
A method and apparatus for determining the enzyme or enzymes in the human body which metabolize a particular drug. Microsomes are obtained from each of several donors. The microsome for one donor is reacted with a test drug and the quantity of metabolites produced is determined. The microsomes are similarly each reacted with the test drug and the quantity of metabolites produced for each microsome is determined to generate drug metabolism data. The drug metabolism data obtained is compared to reference data which indicates the activity of a select number of major enzymes in each of the donors. The reference data for each enzyme is separately tabulated. The enzyme responsible for the metabolism of the test drug is identified when the metabolism data correlates with the tabulated reference data for that enzyme.

REFERENCES:
patent: 4066510 (1978-01-01), Thilly
patent: 5100779 (1992-03-01), Watkins
Schenkman "Cytochrome P450" pp. 503-524, 1993.
Waterman "Methods in Enzymology" vol. 206 pp. 141-190, 1991.
Jerina "Drug Metabolism Concepts" pp. 46-71 & 81-98 1977.
Dybing et al. "In Vitro Metabolism & Activation of Carcinogenic Aromatic Amines by Subcellular Fractions of Human Liver" Cancer Res 39 4206-4211 1979.

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