Process for the long-term surviving culture of hepatocytes

Details Process for the long-term surviving culture of hepatocytes Process for the long-term surviving culture of hepatocytes

1985-06-06

1990-04-03

Warren, Charles F.

Chemistry: molecular biology and microbiology

Spore forming or isolating process

43524021, 43524023, 435240243, C12N 500, C12N 502

Patent

active

049140329

ABSTRACT:
A process is disclosed for the long term surviving culture of hepatocytes maintaining their morphological and functional characteristics for long-term periods, suitable to assest the acute and chronic hepatotoxicity of drugs, chemicals and environmental pollutants. The long term surviving culture of hepatocytes is carried out in a culture media containing fibroblast cells or fibroblast cell products treated to prevent cell multiplication and inoculated under controlled density.

REFERENCES:
Schulte-Hermann, et al, Arch Pharmocol 273, 109-122, 1972, "Selective Inhibition of Livercell Proliferation by CFT 1201 and SKF 525A".
Wolf, K. Methods in Enzymology, vol. 53, 466-474, 1979, "Cold-Blooded Vertebrate Cell and Tissue Culture", Editor: Jakoby W. & Pastan, I.
Freshney, R. I., 1983, Culture of Animal Cells, p. 78.
Michaelopoulos, G. et al. In Vitro 15, 1979 796-806, "Primary Culutures of Hepatocyteson Human Fibroblasts."
Rheinwald, J. Methods in Cell Biology vol.21A, 1980, "Serial Cultivation of Normal Human Epidermal Keratinocytes", 229-254.
Althaus, F. R. Vet. Pharmacol Toxicol, , 1983, 465-478, "Hepatocyte Cultures as a Research Tool in Pharmacology and Toxicology,"
Rheinwald, et al, Cell, 6, 1975, 331-343, "Serial Cultivation of Strains of Human Epidermal Keratirocytes: the Formation of Keratinizing colonies from Single Cells".
Langenbach et al., Cancer Research, vol. 39, p. 3511 (1979).
Gugen-Gillouso et al., Exp. Cell Res., vol. 143, p. 53 (1983).
Althaus, Vet. Pharmacol, Toxicol., pp. 465-477 (1983).

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