Method of determining glucose-6-phosphate and composition theref

Details Method of determining glucose-6-phosphate and composition theref Method of determining glucose-6-phosphate and composition theref

1994-01-26

1996-02-20

Wityshyn, Michael G.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving oxidoreductase

435 25, 435 4, 435 22, 435 17, 435 14, 436 94, 436 95, C12Q 126, C12Q 132, G01N 3300

Patent

active

054928150

ABSTRACT:
A method is provided for determining glucose-6-phosphate, including the step of dehydrogenating glucose-6-phosphate and NAD or NADP in the presence of glucose-6-phosphate dehydrogenase to produce 6-phosphogluconate and NADH or NADPH, wherein the determination is carried out in the presence of 6-phosphogluconolactonase. Also provided is a composition for determining glucose-6-phosphate, characterized by containing 6-phosphogluconolactonase, glucose-6-phosphate dehydrogenase, and NAD or NADP.

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