Manufacture and expression of structural genes

Details Manufacture and expression of structural genes Manufacture and expression of structural genes

1982-05-06

1987-03-24

Wiseman, Thomas G.

Organic compounds -- part of the class 532-570 series

Organic compounds

Carbohydrates or derivatives

536 28, 536 29, 435 68, 435 70, 435 91, 4351723, 435317, 935 16, 935 17, C07H 2104, C07H 2102, C12P 2100, C12P 2102, C12P 1934, C12N 1500, C12N 100

Patent

active

046526390

ABSTRACT:
Described are rapid and highly efficient procedures for the total synthesis of linear, double stranded DNA sequences of up to about 200 base pairs, which sequences may comprise entire structural genes. Illustratively disclosed is the preparation and expression of manufactured genes, including fusion genes, capable of directing synthesis of human .beta.-endorphin and of proteins which differ from human .beta.-endorphin in terms of the identity or relative position of one or more amino acids. Manufactured genes preferably include codons selected from among alternative codons specifying the same amino acid on the basis of preferential expression in a projected host microorganism (e.g., E. coli) to be transformed.

REFERENCES:
patent: 4403036 (1983-09-01), Hartley et al.
Itakura et al: Science 198, 1056 (1977).
Edge et al: Nature 292, 756 (1981).
Grantham et al: Nucleic Acids Res. 8, 1893 (1980).
Kennell: Progr. Nucl. Acid Res. Mol. Biol. 11, 259 (1971).
Doel et al: Nucleic Acids Research 8, 4575 (1980).
"BRL Restriction Endonuclease Reference Chart", appearing in the '81/'82 Catalog of Bethesda Research Laboratories, Inc., Gaithersburg, Md., pp. 164, 165, and 168.
Goeddel, et al., Nucleic Acids Research, 8: 4057-4074 (1980).
Goeddel, et al., Nature, 281: 544-548 (1981).
Aharonowitz, et al., Scientific American, 245(3): 140-152 (1981).

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