Organic compounds -- part of the class 532-570 series – Organic compounds – Heterocyclic carbon compounds containing a hetero ring...
Patent
1992-05-07
1993-05-11
Ivy, C. Warren
Organic compounds -- part of the class 532-570 series
Organic compounds
Heterocyclic carbon compounds containing a hetero ring...
549357, 549452, 564 86, C07D28508, C07D30714, C07D30904, C07D41712
Patent
active
052102033
ABSTRACT:
Nucleic acid hybridization probes are provided which comprise an N.sup.4 -(substituted amino)cytosine moiety, wherein the substituted amino group comprises a tag moiety, whereby the probe is detected. Methods of preparing probes of the invention, intermediates used in such methods, and methods of using the probes of the invention in hybridization assays are also provided. Typical tag moieties employed with the probes of the invention are biotinyl, aminothiadiazole and fluorescein derivatives, connected to N.sup.4 -amino groups of modified cytosines of the probe through linker moieties. Probes tagged with biotin are typically detected by binding to the biotinyl moieties, through a streptavidin or avidin molecule, a reporter group which includes streptavidin or avidin and then detecting a signal due to the reporter group. Probes tagged with aminothiadiazole derivatives are typically detected by essentially the same method as those tagged with biotinyl but employing as reporter group one which binds to the derivative through a carbonic anhydrase molecule. Probes tagged with fluorescein derivatives are detected by a fluorescence spectroscopic method without binding of a reporter group to the tag.
REFERENCES:
patent: 4780405 (1988-10-01), Kaiser et al.
D. E. Draper, "Attachment of Reporter Groups to Specific, Selected Cytidine Residues in RNA Using a Bisulfite-catalyzed Transamination Reaction," Nucl. Acids Res. 12,989-1002(1984).
Goya et al., "Sulfanilamides as Inhibitors of Oxidation of Ammoniacal Nitrogen," Chemical Abstracts, vol. 78, No. 13, Apr. 2, 1973, p. 351, Col. 2, Abstract No. 83315r.
Nitta et al., "A New Reaction Useful for Chemical Cross-Linking Between Nucleic Acids and Proteins," FEBS Letters 166, 194-198 (1984).
Wistrand et al., "Sulphonamide Carbonic Anhydrase Inhibitors and Intra-Ocular Pressure in Rabbits," Acta pharmacol. et toxicol., 17, 337-355 (1960).
Ghosh Soumitra
Kaiser Emil T.
Musso Gary F.
Ivy C. Warren
Siska Diagnostics Inc.
Spivack P. G.
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