Process and reagent for the determination of substrates or enzym

Details Process and reagent for the determination of substrates or enzym Process and reagent for the determination of substrates or enzym

1991-02-26

1993-03-23

Naff, David M.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving oxidoreductase

435 26, 435 28, 435805, 548250, C12Q 126, C12Q 128, C12Q 132

Patent

active

051963140

ABSTRACT:
A process for the determination of substrate or enzyme activities by the use of a redox reaction as a measurement reaction is carried out in the presence of one or more additionally added tetrazolium salts to remove disturbing substances. The tetrazolium salts have the formula ##STR1## in which R.sup.1 is a hydrogen atom, a carboxyl group or an alkyl, phenyl, nitrophenyl, dinitrophenyl, carboxyl-substituted phenyl or trialkylammoniumphenyl radical, R.sup.2 is a phenyl, nitrophenyl, biphenylyl or naphthyl radical, R.sup.3 is a phenyl, carboxyl-substituted phenyl, carboxyl-substituted hydroxyphenyl or dimethylthiazolyl radical, and A.sup..crclbar. is a monovalent anion. The formazanes formed by reaction with reducing substances do not absorb light at all, or absorb light only to a negligible extent, at the measurement wavelength of the redox reaction.
New compounds included within the structural formula are those in which R.sup.1 is carboxy, phenyl, 2-carboxyphenyl, 4-carboxyphenyl, 2,4-dinitrophenyl or 4-trimethylammoniumphenyl, R.sup.2 is phenyl, and R.sup.3 is phenyl or 2-carboxyphenyl; with the proviso that, when R.sup.3 is unsubstituted phenyl or 2-carboxyphenyl, R.sup.1 may not be unsubstituted phenyl.

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