Method of screening for human sperm abnormalities as part of a r

Details Method of screening for human sperm abnormalities as part of a r Method of screening for human sperm abnormalities as part of a r

1991-10-24

1994-10-25

Parr, Margaret

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

435806, 435810, 935 77, C12Q 168

Patent

active

053588479

ABSTRACT:
A method for determining the capacity of a human sperm to fertilize a human egg is described by assessing sperm activation events in an in vitro assay using Xenopus laevis frog egg extract. Fertilizing capacity is assessed as a comparison of sperm decondensation, DNA synthesis and/or sperm recondensation as between a test sperm sample sperm and a sperm sample from a proven fertile human male. The method employs results from the in vitro assay to also determine relative sufficiency or insufficient of a sperm sample for fertilizing a human egg in human couples with a history of a diagnosed "unexplained infertility" from standard infertility diagnostic tests. The method may also be used to screen human sperm donors in human artificial insemination programs. A fixed-slide cytoprep sperm analysis of decondensed sperm chromatin, as between a sperm test sample and a sperm sample from a proven fertile human male, may also be used to confirm in vitro decondensation results of the infertility or fertility of a particular human male. A kit is also provided for testing male sperm samples for human egg fertilizing capacity.

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