Chemistry: molecular biology and microbiology – Treatment of micro-organisms or enzymes with electrical or... – Modification of viruses
Patent
1979-10-22
1982-09-07
Tanenholtz, Alvin E.
Chemistry: molecular biology and microbiology
Treatment of micro-organisms or enzymes with electrical or...
Modification of viruses
435 68, 435235, 435317, C12N 1500
Patent
active
043484778
ABSTRACT:
A method for preparing a novel recombinant DNA, which comprises (1) cleaving with an endonuclease a phage DNA having an endonuclease-sensitive region not in the DNA segment participating in temperate phage DNA replication and integration of DNA into a host chromosome but in other DNA segments, a temperate phage DNA having an endonuclease-sensitive region in the DNA segment carrying genetic information for the production of coat protein, and a DNA carrying the intended genetic information, (2) mixing together all fragments produced by said cleaving, (3) adding DNA ligase to the mixture, and (4) recovering from the resulting mixture a phage DNA having its coat protein producing ability deleted by the replacement of the DNA segment carrying genetic information for coat protein production with a DNA fragment carrying the intended genetic information.
REFERENCES:
Murray et al., Nature, vol. 251, pp. 476-481, Oct. 1974.
Scott et al., Molecular Cloning of Recombinant DNA, pp. 133-153 (1977).
Lewin, Gene Expression-3, pp. 269, 270, 879, 886 & 901 (1977).
Thomas et al., Proc. Nat. Acad. Sci. USA, vol. 71, No. 11, pp. 4579-4583, Nov. 1974.
Fukushima Danji
Masuda Tsutomu
Nakano Eiichi
Saito Narimasa
Noda Institute for Scientific Research
Tanenholtz Alvin E.
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