Process for labeling nucleic acids and hybridization probes

Details Process for labeling nucleic acids and hybridization probes Process for labeling nucleic acids and hybridization probes

1985-10-25

1986-10-14

Nucker, Christine M.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

548303, 435 7, 436 94, 436501, 935 78, C12Q 168, G01N 3348

Patent

active

046172610

ABSTRACT:
Nucleic acids may be labeled by intercalating the alkylating intercalation moiety of a labeling reagent into a partially double-stranded nucleic acid to form a complex and activating the complex to cause covalent bonding between the reagent and the nucleic acid. Preferably, the labeled nucleic acid is a hybridization probe for detecting nucleic acid sequences capable of hybridizing with a hybridizing region of the nucleic acid. Also preferably the label moiety is non-radioactive. The labeling reagent is of the formula:

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