Electrospraying apparatus and method for introducing material in

Details Electrospraying apparatus and method for introducing material in Electrospraying apparatus and method for introducing material in

1998-06-05

2000-07-25

Weber, Jon P.

Chemistry: molecular biology and microbiology

Treatment of micro-organisms or enzymes with electrical or...

C12N 1300

Patent

active

06093557&

ABSTRACT:
A method of introducing biological material into cells includes providing one or more target cells and establishing a spray of substantially dispersed particles including biological material. The substantially dispersed particles have an electrical charge applied thereto such that one or more of the substantially dispersed particles of the spray is introduced into one or more of the target cells. The spray of substantially dispersed particles may be established by dispensing a spray of microdroplets suspending particles. The electrical charge is concentrated on the suspended particles as the microdroplet evaporates. The suspended particles may include carrier particles with biological material or the suspended particles may be particles of biological material alone. The space charge effect of the concentrated electrical charge on the substantially dispersed particles of the spray enable one or more of the particles to be introduced into one or more of the target cells.

REFERENCES:
patent: 4795330 (1989-01-01), Noakes et al.
patent: 4945050 (1990-07-01), Sanford et al.
patent: 5036006 (1991-07-01), Sanford et al.
patent: 5066587 (1991-11-01), Jones et al.
patent: 5100792 (1992-03-01), Sanford et al.
patent: 5120657 (1992-06-01), McCabe et al.
patent: 5141131 (1992-08-01), Miller, Jr. et al.
patent: 5149655 (1992-09-01), McCabe et al.
patent: 5179022 (1993-01-01), Sanford et al.
patent: 5204253 (1993-04-01), Sanford et al.
patent: 5219746 (1993-06-01), Brinegar et al.
patent: 5240842 (1993-08-01), Mets
patent: 5247842 (1993-09-01), Kaufman et al.
patent: 5371015 (1994-12-01), Sanford et al.
patent: 5457041 (1995-10-01), Ginaven et al.
patent: 5478744 (1995-12-01), Sanford et al.
patent: 5506125 (1996-04-01), McCabe et al.
patent: 5516670 (1996-05-01), Kuehnle et al.
patent: 5525510 (1996-06-01), McCabe et al.
patent: 5584807 (1996-12-01), McCabe
Adachi et al., "High-efficiency unipolar aerosol charger using a radioactive alpha source," Aerosol Science, Idustry Health and Environment, pp. 439-441, (Matsuda and Takahashi, eds.), Pergamon Press, NY (1990).
Buscher et al., "Performance of a unipolar `square wave` diffusion charger with variable nt-product," J. Aerosol Sci., 25(4), 651-663 (1994).
Chen et al., "Electrospraying of Conductor Liquids for Monodisperse Aerosol Generation in the 4nm to 1.8 .mu.m Diameter Range," J. Aerosol Sci., 26, 963-977 (1995).
Chen et al., "Experimental Investigation of Scaling Laws for Electrospraying: Dielectric Constant Effect," Aerosol Science and Technology, 27, 367-380 (1997).
Fuchs, "On the Stationary Charge Distribution on Aerosol Particles in a Bipolar Ionic Atmosphere," Geodis:Pura. Appl., 56, 185-193 (1963).
Lui et al., "On unipolar diffusion charging of aerosol particles in the continuum regime," J. Colloid Interface Sci., 58, 142-149 (1977).
Pui et al., "Nanometer Particles: A New Frontier For Multidisciplinary Research," J. Aerosol Sci., 28(4), 539-544 (1997).
Pui et al., "Unipolar Diffusion Charging Ultrafine Aerosols," Aerosol Sci. Techn., 8, 173-187 (1988).
Romay et al., "Unipolar Diffusion Charging of Aerosol Particles at Low Pressure," Aerosol Sci Techn., 15, 60-68 (1991).
Romay et al., On the combination coefficient of positive ions with ultrafine neutral particles in the transition and free-molecule regimes, Aerosol Sci. Techn., 17, 134-147 (1992).
Romay et al., "Free electron charging of ultrafine aerosol particles," J. Aerosol Sci., 23(7), 679-692 (1992).
Wiedensohler et al., "A novel unipolar charger for ultrafine aerosol particles with minimal particle losses" J. Aerosol Sci., 25(4), 639-650 (1994).

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