Method for instrument determination of a measured variable which

Details Method for instrument determination of a measured variable which Method for instrument determination of a measured variable which

1997-09-24

2000-03-28

Hoff, Marc S.

Data processing: measuring, calibrating, or testing

Measurement system in a specific environment

Chemical analysis

436517, 356341, G01N 3100, G01N 3300

Patent

active

060443308

ABSTRACT:
The present invention relates to a method for instrument determination of measured variable L(t) which changes with time, the intention being to determine the maximum value of L(t) in a region with a linear reaction profile, and the time as well as the duration of the reaction time window being variable with the linear region and depending on the nature of the reaction and the reaction conditions. Specifically, the present invention relates to the determination of protein concentrations with the aid of light scatter, which is produced by specific antibodies, in homogeneous solutions. In particular, the invention relates to reactions which take place slowly and have a largely linear profile over a relatively long time, the rate of formation of antigen-antibody complexes in the linear section of the reaction (V.sub.MaxLin) being determined as the measured variable.

REFERENCES:
patent: 3990851 (1976-11-01), Gross et al.
patent: 4157871 (1979-06-01), Anderson et al.
patent: 4581337 (1986-04-01), Frey et al.
patent: 5244815 (1993-09-01), Guirguis
patent: 5635602 (1997-06-01), Cantor et al.
patent: 5705353 (1998-01-01), Oh et al.

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