Chemistry: molecular biology and microbiology – Enzyme – proenzyme; compositions thereof; process for... – Oxidoreductase
Patent
1997-03-28
1999-11-16
Fox, David T.
Chemistry: molecular biology and microbiology
Enzyme , proenzyme; compositions thereof; process for...
Oxidoreductase
435 697, 435 698, 530370, C12N 906, C12N 1529, C12N 1553, C12N 1562
Patent
active
059856343
ABSTRACT:
Amino acid and nucelotide sequences relating to the glutamate dehydrogenase (GDH) enzyme are described. The GDH enzymes descnred herein were discovered in the alga Chlorella sorokinina in the form of seven different inducible isoenzymes. These isoenzymes are found in the algae as chloroplast-localized hexamers composed of .alpha.- and .beta.-subunits. Plants transfromed with nucleotide sequences encoding the .alpha.- or .beta.-subunits of the enzyme show imrpoved properties, for example, increased growth and improved stress tolerance.
REFERENCES:
Miller et al. Plant Mol. Biol. 0: 1-21, 1998.
Cock, J.M. et al. (1991) "A nuclear gene with many introns encoding ammonium-inducible chloroplastic NADP-specific glutamate dehydrogenase(s) in Chlorella sorokiniana" Plant Moledular Biology 17:1023-1044.
Bascomb, N.F. et al. (1987) "Different Rates of Synthesis and Degradation of Two Chloroplastic Ammonium-inducible NADP-Specific Glutamate Dehydrogenase Isoenzymes during Induction and Deinduction of Chlorella sorokiniana Cells" Plant Physiol. 83:85-91.
Bascomb, N.F., R. R. Schmidt (1987) "Purification and Partial Kinetic and Physical Characterization of Two Chloroplast-Localized NADP-Specific Glutamate Dehydrogenase Isoenzymes and Their Preferential Accumalation in Chlorella sorokiniana Cells Cultured at Low or High Ammonium Levels" Plant Physiol. 83:75-84.
Prunkard, D.E. et al. (1986) "Effect of Different Carbon Sources on the Ammonium Induction of Different Forms of NADP-Specific Glutamate Dehydrogenase in Chlorella sorokiniana Cells Cultured in the Light and Dark" Plant Physio. 81:413-422.
Yeung, A.T. et al. (1981) "Purification of an Ammonium-Inducible Glutamate Dehydrogenase and the Use of its Antigen Affinity Column-Purified Antibody in Specific Immunoprecipitation and Immunoadsorption Procedures" Analytical Biochemistry 110:216-228.
Meredith, M.J. et al. (1978) "Physical and Kinetic Properties of the Nicontinamide Adenine Dinucleotide-specific Glutamate Dehydrogenase Purified from Chlorella sorokiniana" Plant Physio. 61:967-974.
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Prunkard, D. E. et al. (1986) "Evidence for Chloroplastic Localization of an Ammonium-Inducible Glutamate Dehydrogenase and Synthesis of its Subunit from a Cytosolic Precursor-Protein in Chlorella sorokiniana" Plant Physio. 81:349-355.
Wallsgrove, R.M. et al. (1987) "Barley Mutants Lacking Chloroplast Glutamine Synthetase-Biochemical and Genetic Analysis" Plant Physio. 83:155-158.
Miflin, B.J. P. J. Lea (1979) "The Pathway of Nitrogen Assimilation in Plants" Phytochemistry 15:873-885.
"Niotinasmide Adenine Dl Nucleotide Glutamate Dehydrogenase Obtain Chlorella Cell Buffer Extract Two Stage Chromatography Phosphate Buffer Elution" (1982) Biochem. Inst., **abstract only.In.
Napoli et al. Introduction of a chimeric chalcone synthase gene into petunia results lin reversible co-suppression of homologous genes in trans. The Plant Cell. 2:279-289 (1990).
Bascomb, N.F. et al. (1986) "Specific Polysome Innunoadsorption to Purify an Ammonium-Inducible Glutamate Dehydrogenase MRNA from Chlorella sorokiniana and synthesis of Full Length Double-Stranded cDNA from the Purified MRNA" Plant Physio. 81:527-532.
Miller, P. W. et al. (1994) "Transcription initiation site of a NADP-specific glutamate dehydrogenase gene and potential use of its promoter region to express foreign genes in ammonium-cultured Chlorella sorokiniana cells" Journal of Applied Phycology 6:211-223.
Meredith, M. J. R.R. Schmidt (1991) "NAD-Specific glutamate dehydrogenase isoenzyme localized in mitochondria of nitrate-cultured Chlorella sorokinian cells" Plant Physio. 10:67-71.
Miller Philip
Schmidt Robert R.
Fox David T.
University of Florida Research Foundation Inc.
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