DNA sequencing method and DNA sample preparation method

Details DNA sequencing method and DNA sample preparation method DNA sequencing method and DNA sample preparation method

1996-09-18

1999-11-16

Horlick, Kenneth R.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

435 911, 435 912, 536 243, 536 2433, C12Q 168, C12P 1934, C07H 2104

Patent

active

059855568

ABSTRACT:
A kit and a method of DNA sequencing including digesting a sample DNA with a restriction enzyme to obtain a DNA fragment; introducing an oligonucleotide having a definite base sequence into the DNA fragment at the 3' terminus; and performing a complementary strand extension reaction, using a labeled primer. The complementary strand extension reaction uses as a template, a single strand of the DNA fragment having the oligonucleotide introduced thereinto, to obtain a labeled extended primer having a base sequence complementary to the single strand of the DNA fragment. Next a sequencing reaction with the labeled primer and using the single strand of the DNA fragment having the oligonucleotide introduced thereinto, is performed with the extended labeled primer, using as a template, a part of the single strand of the sample DNA having the base sequence of the single strand of the DNA fragment and a contiguous sequence adjacent thereto, or a single strand of the sample DNA. Finally the products of the sequencing reaction are electrophoresed to determine the base sequence of the DNA fragment and at least a part of the base sequence of the sample DNA adjacent to the base sequence of the DNA fragment.

REFERENCES:
patent: 5650274 (1997-07-01), Kambara et al.
patent: 5817464 (1998-10-01), Kambara et al.
patent: 5824481 (1998-10-01), Kambara et al.
patent: 5861252 (1999-01-01), Kambara et al.
Okano et al., Gene 176, 231-235 (1996).
Surikagaku, No. 359, May (1993), pp. 74-81.
Sambrook et al, Molecular Cloning, 2nd Ed. Cold Spring Harbor Laboratory Press (1989), pp. 13-21 -13-23.
Kieleczawa et al Science, 258 (1992), pp. 1787-1791.
Studier, Proc. Natl. Acad. Sci. U.S.A., 86, 6917-6921 (1989).
Furuyama et al. DNA Research, 1, 231-237 (1994).

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