Method of reconstructing animal organs

Details Method of reconstructing animal organs Method of reconstructing animal organs

1998-07-09

1999-11-16

Saucier, Sandra

Chemistry: molecular biology and microbiology

Differentiated tissue or organ other than blood, per se, or...

Including perfusion; composition therefor

435 11, A01N 102

Patent

active

059855398

ABSTRACT:
The present invention relates to a method for reconstruction of animal organ which comprises perfusing a cell-dispersible solution to animal organ from vascular system and then perfusing a cell culture medium whereby the organ is reconstructed.
According to the present invention, the organ can be reconstructed and cultured for long term without separating most of cells which constitute the animal organ.

REFERENCES:
Vaananen et al., "Selective Isolation of Intact Peri-portal or PeriOvenous Hepatocytes by Anterograde or Retrograde Coallagenase Gradient Perfusion", Liver 3 (3) : 131-139 (1983).
J. Gerlach, et al "High Yield hepatocyte isolation from pig livers for investigation of hybrid liver support systems: influence of collagenase concentration and body weight" Journal of Surgical Research, vol. 62, 1996, pp. 85-89.
B.D. Foy et al: "Engineering organ perfusion protocols: NMR analysis of hepatocyte isolation from perfused rat liver" Biotechnology and Bioengineering, vol. 43, 1994, pp. 661-672.
M.E. Pueyo, et al: "A method for obtaining monodispersed cells from isolated porcine islets of Langerhans" The International Journal of Artificial Organs, vol. 18, No. 1, 1995.
M. Takahashi, et al: "Isolation and culture of human hepatocytes from resected liver tissue as a bioreactor for a hybrid artificial liver" Artificial Organs, vol. 17, No. 7, 1993, pp. 653-659.
A. K. Campbell, et al: "Maintenance of Viable Cells in an Organ Culture of Mature Rat Liver" Experimental Cell Research 68, (1971) pp. 33-42.
D. W. John, et al: "Effect of Aflatoxin B1 on Net Synthesis of Albumin, Fibrinogen, and .alpha.1 -Acid Clycoprotein by the Isolated Perfused Rat Liver" Biochemical Pharmacology, vol. 18, pp. 1135-1146; GB.
D. W. John, et al: "Influence of Actinomycin D and Puromycin on Net Synthesis of Plasma Albumin and Fibrinogen by the Isolated Perfused Rat Liver" The Journal of Biological Chemistry, vol. 241, No. 21, Issue of Nov. 10, pp. 4817-4824, (1966).

Affiliated with

Also associated with

Rating

Method of reconstructing animal organs does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Method of reconstructing animal organs, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Method of reconstructing animal organs will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-1322779