Cryopreservation and cell culture medium comprising less than 50

Details Cryopreservation and cell culture medium comprising less than 50 Cryopreservation and cell culture medium comprising less than 50

1997-08-01

1999-11-16

Saucier, Sandra E.

Chemistry: molecular biology and microbiology

Differentiated tissue or organ other than blood, per se, or...

435 13, 435 2, A01N 102

Patent

active

059855380

ABSTRACT:
A cell culture medium and cryopreservation medium in which sodium chloride is replaced with an organic cation, preferably choline chloride in a concentration of at least 100 mM, resulting a residual sodium ion concentration less than about 50 mM. The cryopreservation solution is suitable for cryopreservation of unfertilized oocytes, with thawed oocytes demonstrating the ability to survive, fertilize, and for the resulting embryos to proceed to full term development.

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