Process for producing human epidermal growth factor and analogs

Details Process for producing human epidermal growth factor and analogs Process for producing human epidermal growth factor and analogs

1986-02-24

1988-05-10

Kight, John

Chemistry: natural resins or derivatives; peptides or proteins;

Proteins, i.e., more than 100 amino acid residues

530324, 935 47, 935 48, C07K 1300, C07K 710, C12N 1500

Patent

active

047436794

ABSTRACT:
Disclosed is a process for producing a fusion protein comprising epidermal growth factor (EGF) attached through a Glu-residue to a leader. Treatment of the fusion protein with a Staphylococcus aureus V8 protease specific for cleaving peptides at a Glu-linkage produces large amounts of the 53 amino acid EGF sequence.
The treatment conditions optimize production of the 53 and 51 amino acid EGFs relative to shorter analogs, and take full advantage of the selectivity of the protease.

REFERENCES:
patent: 3917824 (1975-11-01), Camble et al.
patent: 4338397 (1982-07-01), Gilbert et al.
Brake et al., Proc. Natl. Acad. Sci., USA, vol. 81, pp. 4642-4646 (1984).
Hogg, The Journal of Biological Chemistry, vol. 256, No. 4, pp. 1935-1939 (1981).

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