Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Recombinant dna technique included in method of making a...
Patent
1997-04-14
1999-07-20
Ulm, John
Chemistry: molecular biology and microbiology
Micro-organism, tissue cell culture or enzyme using process...
Recombinant dna technique included in method of making a...
435 691, 4352523, 4353201, 530350, 536 234, C07K 14705, C07K 1628, C12N 1562
Patent
active
059255498
ABSTRACT:
Chimeric polypeptides containing the N-terminal amino acid sequence of a glycoprotein hormone receptor polypeptide and a membrane anchor polypeptide, with a protease recognition site between the two, are disclosed. Also disclosed are nucleic acids encoding such polypeptides, expression vectors containing such nucleic acids and methods of producing such recombinant chimeric polypeptides, as well as uses thereof. The chimeric polypeptides are particularly useful for the production of soluble glycoprotein hormone receptor polypeptides.
REFERENCES:
patent: 5359046 (1994-10-01), Capon et al.
patent: 5447851 (1995-09-01), Beutler et al.
patent: 5494806 (1996-02-01), Segre et al.
Benkirane, M. et al., "The cytoplasmic domain of CD4 plays a critical role during the early stages of HIV infection in T-cells," The EMBO Journal 13(23):5559-5569 (1994).
Jelinek, L.J., et al., "Expression Cloning and Signaling Properties of the Rat Glucagon Receptor," Science 259:1614-1616 (1993).
Lin, C. et al., "Pit-1-dependent expression of the receptor for growth hormone releasing factor mediates pituitary cell growth," Nature 360:756-768 (1992).
Masu, M. et al., "Sequence and expression of a metabotropic glutamate receptor," Nature 349: 760-765 (1991).
Thorens, B. et al., "Cloning and Functional Expression of the Human Islet GLP-1 Receptor," Diabetes 42: 1678-1682 (1993).
Chen, W. and Bahl, O.P. (1993) "High Expression of the Hormone Binding Active Extracellular Domain (1-294) of Rat Lutropin Receptor in Escherichia coli", Molecular and Cellular Endocrinology, 91:35-41.
Chen, J. et al. (1994) "Confirmation of the Intramolecular Tethered Liganding Hypothesis and Discovery of an Alternative Intermolecular Liganding Mode", The Journal of Biological Chemistry, 269(23):16041-16045.
Deng, H. et al. (1996) "Identification of a Major Co-Receptor for Primary Isolates of HIV-1", Nature, 381:661-666.
Feng, Y. et al. (1996) "HIV-1 Entry Cofactor: Functional cDNA Cloning of a Seven-Transmembrane, G Protein-Coupled Receptor", Science, 272:872-877.
Ji, I. and Ji, T.H. (1991) "Exons 1-10 of the Rat LH Receptor Encode a High Affinity Hormone Binding Site and Exon 11 Encodes G-Protein Modulation and a Potential Second Hormone Binding Site", Endocrinology, 128(5):2648-2650.
Ji, I. and Ji, T.H. (1991) "Human Choriogonadotropin Binds to a Lutropin Receptor With Essentially No N-Terminal Extension and Stimulates cAMP Synthesis", The Journal of Biological Chemistry, 266(20):13076-13079.
Loosfelt, H. et al. (1989) "Cloning and Sequencing of Porcine LH-hCG Receptor cDNA: Variants Lacking Transmembrane Domain ", Science, 245:525-528.
McFarland, K.C. et al. (1989) "Lutropin-Choriogonadotropin Receptor : An Unusual Member of the G Protein-Coupled Receptor Family", Science, 245:494-499.
Tsai-Morris, C.H. et al. (1990) "Intronic Nature of the Rat Luteinizing Hormone Receptor Gene Defines a Soluble Receptor Subspecies with Hormone Binding Activity", The Journal of Biological Chemistry, 265(32):19385-19388.
Tsai-Morris, C.H. et al. (1991) "Structural Organization of the Rat Luteinizing Hormone (LH) Receptor Gene", The Journal of Biological Chemistry, 266(17):11355-11359.
VuHai-LuuThi, M.T. et al. (1992) "Variant Forms of the Pig Lutropin/Choriogonadotropin Receptor", Biochemistry, 31:8377-8383.
Braun, T., et al., "Amino-Terminal Leucine-Rich Repeats in Gonadotropin Receptors Determine Hormone Selectivity," EMBO Journal. 10(7):1885-1890 (1991).
Ji, I. and Ji, T.H., "Differential Roles of Exoloop 1 of the Human Follicle-stimulating Hormone Receptor in Hormone Binding and Receptor Activation," Journal of Biological Chemistry. 270(27):15970-15973 (1995).
Thomas, D.M. and Segaloff, D.L., "Hormone-Binding Properties and Glycosylation Pattern of a Recombinant Form of the Extracellular Domain of the Luteinizing Hormone/Chorionic Gonadotropin Receptor Expressed in Mammalian Cells," Endocrinology. 135(5):1902-1912 (1994).
Vilardaga, J.-P., "Properties of Chimeric Secretin and VIP Receptor Proteins indicate the Importance of the N-Terminal Domain for Ligand Discrimination," Biochemical and Biophysical Research Communications. 211(3):885-891 (1995).
Xie, Y.-B., et al., "Extracellular Domain of Lutropin/Choriogonadotropin Receptor Expressed in Transfected Cells Binds Choriogonadotropin with High Affinity," Journal of Biological Chemistry. 265(35):21411-21414 (1990).
Hsueh Aaron J. W.
Kobilka Brian K.
Kudo Masataka
Petithory Joanne R.
Sholtz Charles K.
The Board of Trustees of the Leland Stanford Junior University
Ulm John
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