Intermittent collection of mononuclear cells in a centrifuge app

Details Intermittent collection of mononuclear cells in a centrifuge app Intermittent collection of mononuclear cells in a centrifuge app

1997-06-09

1999-03-09

Cooley, Charles E.

Imperforate bowl: centrifugal separators

With means for indicating, inspecting, measuring, signaling...

494 45, B04B 1104

Patent

active

058792805

ABSTRACT:
A centrifuge apparatus is used for collecting white blood cells, primarily mononuclear cells, from whole blood stratified into layers. A thin mononuclear (MNC) layer is formed at the interface of red blood cells and plasma. A barrier is positioned in the separation vessel of the centrifuge at a location to intercept the thin layer. An MNC collect port is positioned in front of the barrier to collect the thin layer. MNC fluid is allowed to pool behind the barrier to surround the collect port before collection is started. Collection ceases when the pool is removed and allowed to build again. By operating the collect in an intermittent fashion, improvements in purity and collect volume are achieved. The intermittent collection procedure can be useful for harvesting granulocytes and, in general, any sparse stratified component of a centrifuged solution where the sparse component is layered between more dense and less dense strata.

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