Stabilized thymidine phosphorylase preparation and culture mediu

Details Stabilized thymidine phosphorylase preparation and culture mediu Stabilized thymidine phosphorylase preparation and culture mediu

1978-06-13

1980-08-26

Warden, Robert J.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving viable micro-organism

435194, 435253, C12Q 118, C12N 912, C12N 120

Patent

active

042196213

ABSTRACT:
Thymidine phosphorylase counteracts antagonists to antifolate agents in media used for testing the susceptibility of bacteria to anti-folate antimicrobial agents. A solution of thymidine phosphorylase may be stabilized by addition of inorganic phosphate and uracil, the stabilization being most effective at pH7. Furthermore, bovine serum albumin is added to prevent loss of enzymic activity on filtration. The solution may be kept sterile by addition of sodium or potassium azide.

REFERENCES:
patent: 4097337 (1978-06-01), Krenitsky et al.

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