Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid
Patent
1993-01-14
1996-01-09
Jones, W. Gary
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving nucleic acid
435 911, 536 231, 536 243, 536 2433, 536 253, 536 2532, 935 1, 935 2, 935 4, 935 16, 935 19, 935 76, 935 77, C12Q 168, C12P 1934, C07H 1700, C12N 1500
Patent
active
054828368
ABSTRACT:
The invention provides a method for purifying or isolating double stranded DNA intact using triple helix formation. The method includes the steps of complexing an oligonucleotide and double stranded DNA to generate a triple helix and immobilization of the triple helix on a solid phase by means of a molecular recognition system such as avidin/biotin. The purified DNA is then recovered intact by treating the solid phase with a reagent that breaks the bonds between the oligonucleotide and the intact double stranded DNA while not affecting the Watson-Crick base pairs of the double helix. The present invention also provides a method for purifying or isolating double stranded DNA intact by complexing the double stranded DNA with a specific binding partner and recovering the complex during electrophoresis by immobilizing it on a solid phase trap imbedded in an electrophoretic gel.
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Cantor Charles R.
Ito Takashi
Smith Cassandra L.
Jones W. Gary
Sisson Bradley L.
The Regents of the University of California
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