Flying spot fluoro-meter for oxidized flavoprotein and reduced p

Details Flying spot fluoro-meter for oxidized flavoprotein and reduced p Flying spot fluoro-meter for oxidized flavoprotein and reduced p

1978-05-23

1979-07-24

Willis, Davis L.

Radiant energy

Luminophor irradiation

With ultraviolet source

424 7, G01N 2138

Patent

active

041624050

ABSTRACT:
A method and apparatus for measuring the heterogeneity of oxygen delivery to perfused and in situ organs. A laser is employed as a flying spot scanning excitation fluorometer source which provides excitation for either oxidized flavoprotein of the mitochondrial space or reduced nucleotide of mitochondrial and cytosolic spaces. Emission from the two fluorochromes is acquired at 10.sup.4 to 10.sup.5 data points per sec. and histograms of the fluorescence intensity versus the number of occurrences of that intensity are displayed. The histograms show alterations of the intensity and the degree of heterogeneity of the redox states of the organ tissue under study.

REFERENCES:
patent: 3449571 (1969-06-01), Hoerman
patent: 3657537 (1972-04-01), Wheeless, Jr. et al.
patent: 3770349 (1973-11-01), Legorreta-Sanchez

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