Method of making monoclonal antibodies

Details Method of making monoclonal antibodies Method of making monoclonal antibodies

1981-01-26

1984-01-24

Tanenholtz, Alvin E.

Drug, bio-affecting and body treating compositions

Lymphokine

424 88, 435 68, 435172, 435240, 435948, C12P 2100, A61K 3900, C12N 500

Patent

active

044276532

ABSTRACT:
Methods featuring, in one aspect, a method of preparing an antigen or mixture of antigens substantially free of antigens specific to at least two monoclonal antibodies, said method involving contacting an antigen mixture with one or more previously-isolated monoclonal antibodies to form complexes between those antibodies and antigens present in the mixture specific to the antibodies, removing the complexes from the antigen mixture to yield a partially purified antigen mixture, immunizing an animal with the partially purified antigen mixture, fusing spleen cells from the immunized animal to myeloma cells to form hybridomas capable of producing additional monoclonal antibodies, culturing said hybridomas to produce said additional monoclonal antibodies, contacting a sample of the partially purified antigen mixture with said additional monoclonal antibodies to form complexes between said additional monoclonal antibodies and antigens present in the antigen mixture specific to the additional monoclonal antibodies, and removing the complexes from the antigen mixture.

REFERENCES:
Pearson et al., "Dissection of Complex Antigenic Mixtures Using Monoclonal Antibodies and Two-Dimensional Gel Electrophoresis", Analytical Biochemistry, vol. 101, (Jan. 15, 1980), pp. 377-386.
Hurn et al., "Production of Reagent Antibodies", Methods in Enzymology, vol. 70 (1980), pp. 135-142.
Mellman et al., "Purification of a Functional Mouse Fc Receptor Through the Use of a Monoclonal Antibody", Journal of Experimental Medicine, (Oct. 1980), pp. 1048-1049.
Milstein, "Monoclonal Antibodies", Scientific American, (Oct. 1980), pp. 73-74.
Springer, "Monoclonal Antibody Analysis of Complex Biological Systems", Journal of Biological Chemistry 256(8), (1981), pp. 3833-3839.
Anderson et al., "Isolation of Tumor-Associated Macromolecules Using Immunosubtraction", Federation Proceedings 32(3), (1973), Abst. #4165.
Middleton et al., Fed. Proc. (Abstr.), vol. 39, 3464 (1980).
Kennett et al., Top. Micro. Immunol., vol. 81, 77 et seq. (1978).

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