Protein specific fluorescent microspheres for labelling a protei

Stock material or miscellaneous articles – Coated or structually defined flake – particle – cell – strand,... – Particulate matter

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428407, 428500, 521 56, 2601125R, 260117, 260 8, 424 8, 424 85, 424 88, 424 12, 25230135, 525 541, 526268, B32B 516, B32B 904, G01N 3100, G01N 3300

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043260080

ABSTRACT:
Highly fluorescent, stable and biocompatible microspheres are obtained by copolymerizing an acrylic monomer containing a covalent bonding group such as hydroxyl, amine or carboxyl, for example, hydroxyethylmethacrylate, with an addition polymerizable fluorescent comonomer such as dansyl allyl amine. A lectin or antibody is bound to the covalent site to provide cell specificity. When the microspheres are added to a cell suspension the marked microspheres will specifically label a cell membrane by binding to a specific receptor site thereon. The labeled membrane can then be detected by fluorescence of the fluorescent monomer.

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