Simultaneous immunoassay of two analytes using dual enzyme label

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...

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435 792, 435973, 435975, 436500, 436518, G01N 33535

Patent

active

051088967

ABSTRACT:
The present invention relates to dual analyte enzyme immunoassays for assaying two antigens in a single liquid sample wherein the two immunoreactions are carried out simultaneously and wherein subsequently the two enzyme reactions occur simultaneously. Suitable enzyme/substrate pairs are beta-galactosidase
itrophenyl-beta-D-galactoside (p- an/or o-) and alkaline phosphatase/phenolphthalein monophosphate.

REFERENCES:
patent: 4690890 (1987-09-01), Loor et al.

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