Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid
Patent
1999-05-19
2000-08-29
Guzo, David
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving nucleic acid
536 231, 536 243, C07H 2104, C12Q 168
Patent
active
061106860
ABSTRACT:
The present invention provides a human cystatin-like protein (CSTIN) and polynucleotides which identify and encode CSTIN. The invention also provides genetically engineered expression vectors and host cells comprising the nucleic acid sequences encoding CSTIN and a method for producing CSTIN. The invention also provides for agonists, antibodies, or antagonists specifically binding CSTIN, and their use, in the prevention and treatment of diseases associated with expression of CSTIN. Additionally, the invention provides for the use of antisense molecules to polynucleotides encoding CSTIN for the treatment of diseases associated with the expression of CSTIN. The invention also provides diagnostic assays which utilize the polynucleotide, or fragments or the complement thereof, and antibodies specifically binding CSTIN.
REFERENCES:
Rochefort, H., "Biological and clinical significance of cathepsin D in breast cancer," Acta Oncol, 31:125-30 (1992).
Chambers, A., "Ras-Responsive Genes and Tumor Metastasis", Critical Reviews in Oncogenesis, 4(2):95:114 (1993).
Jean, D., et al., "A cysteine proteinase, which cleaves human C3, the third component of complement, is involved in tumorigenicity and metastasis of human melanoma," Cancer Res., 56:254-258 (1996).
Ryan, R.E., "Microglial Cathepsin B: An Immunological Examination of Cellular and Secreted Species," Journal of Neurochemistry, 65:1035-1045 (1995).
Mantle, D., et al., "Comparison of cathepsin protease activities in brain tissue from normal cases and cases with Alzheimer's disease, Lewy body dementia, Parkinson's disease ahd Huntington's disease," Journal of the Neurological Sciences, 131:65-70 (1995).
Huet, G., et al., "Measurement of elastase and cysteine proteinases in synovial fluid of patients with rheumatoid arthritis, sero-negative spondylarthropathies, and osteoarthritis," Clin Chem, 38:1694-7 (1992).
Lenarcic, B., et al., "Human cathepsin B and cysteine proteinase inhibitors (CPIs) in inflammatory and metabolic joint diseases," Biol. Chem Hoppe Seyler 369:257-61 (1988).
Murphy, G., "The regulation of connective tissue metalloproteinases by natural inhibitors," Agents Actions Suppl., 35:69-79 (1991).
Calkins, C., et al., "Mammalian cysteine protease inhibitors: biochemical properties and possible roles in tumor progression," Biol Chem Hoppe Seyler, 376:71-80 (1995).
Chambers et al, "Increased expression of cathepsins L and B and decreased activity of their inhibitors in metastatic, ras-transformed NIH 3T3 cells" Mol. Carcinoq., 5:238-45 (1992).
Rozhin, J., et al., "Cathepsin B to Cysteine Proteinase Inhibitor Balance in Metastatic Cell Subpopulations Isolated from Murine Tumors," Cancer Research, 50:6278-6284 (1990).
Brzin, J. et al., "Human Cystatin, A New Protein Inhibitor of Cysteine Proteinases," Biochemical and Biophysical Research Communications, 118 (1):103-109 (1984).
Abrahamson, M., et al., "Structure and expression of the human cystatin C gene," Biochem J., 268:287-294 (1990).
Schapiro, J., et al., "The effect of high-dose saquinavir on viral load and CD4+ T-cell counts in HIV infected patients," Ann. Intern. Med., 124:1039-1050 (1996).
Stoka, V., et al., "Inhibition of cruzipain, the major cysteine proteinase of the protozoan parasite, Trypanosoma cruzi, by proteinase inhibitors of the cystatin superfamily," FEBS Lett, 370:101-104 (1995).
Vonderfecht, S., et al., "Protease inhibitors suppress the in vitor and in vivo replication of rotavirus," J. Clin Invest, 82:2011-2016 (1988).
Collins, A., et al., "Inhibitory effects of recombinant human cystatin C on human coronaviruses," Antimicrob Agents Chemother, 35:2444-6 (1991).
Saitoh, E., et al. "The human cystatin C gene (CST3) is a member of the cystatin gene family which is localized on chromosome 20," Biochem Biophys Res Commun, 15:162(3):1324-1331 (1989) (GI 181387).
Colella, R., et al., "Chicken Egg White Cystati," The Journal of Biological Chemistry, 264(29)17164-17169 (1989) (GI 118195).
Hall, A., et al., "Structural basis for the biological specificity of cystatin C. Identification of leucine 9 in the N-terminal binding region as a selectivity-conferring residue in the inhibition of mammalian cysteine peptidases," J. Biol. Chem, 270(10)5115-5121 (1995).
Saitoh, E., et al., (GI 181387) GenBank Sequence Database (Accession M27889), National Center for Biotechnology Information, National Library of Medicine, Bethesda, Maryland 2084 (1989).
Colella, R., et al., (GI 118195), GenBank Sequence Database (Accession P01038), National Center for Biotechnology Information, National Library of Medicine, Bethesda, Maryland 2084 (1986).
Colella, R., et al. (GI 118195), GenBank Sequence Database (Accession J05077)(1989).
EMBL/GenBank databases, Accession No. N47763, Sequence Reference HS763285, Feb. 18, 1996.
EMBL/GenBank Databases, Accession No. AA089317, Sequence Reference MMAA89317, Oct. 25, 1996.
Database EST-STS on MPSRCH, accession No. n47763 Feb. 4, 1996.
Bandman Olga
Goli Surya K.
Guzo David
Incyte Pharmaceuticals Inc.
Muenzen Colette C.
Wang Andrew
LandOfFree
DNA hybridizing to a human cystatin-like protein (CSTIN) does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with DNA hybridizing to a human cystatin-like protein (CSTIN), we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and DNA hybridizing to a human cystatin-like protein (CSTIN) will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-1248442