Chemistry: analytical and immunological testing – Biospecific ligand binding assay
Patent
1988-06-09
1991-07-02
Wax, Robert A.
Chemistry: analytical and immunological testing
Biospecific ligand binding assay
435 6, 435 71, G01N 33566, G01N 3353, C12Q 100, C12Q 168
Patent
active
050285459
ABSTRACT:
In a biospecific multianalyte assay the use of microspheres and fluorescent labels with substantially different fluorescence decay times, is combined. The assay is performed in a suspension of microspheres in the form of a pool of different microsphere categories, where the categories represent different analytes. The microspheres belonging to the respective categories are first coated with a specific reactant, i.e. the microspheres function as a solid support for the reactant and for a biospecific reaction. Fluorescent labels having a short decay time are used to identify the category of each individual microsphere, while fluorescent labels having a long decay time are used to determine the concentration of a particular analyte on the microsphere by means of the biospecific reaction.
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Philpitt Fred
Sisson Bradley L.
Wallac Oy
Wax Robert A.
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