Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Preparing compound containing saccharide radical
Patent
1994-08-02
1996-10-15
Lilling, Herbert J.
Chemistry: molecular biology and microbiology
Micro-organism, tissue cell culture or enzyme using process...
Preparing compound containing saccharide radical
435 97, 435193, 435194, 435195, 435200, 435201, 53612313, C12P 1912, C12N 910
Patent
active
055653413
ABSTRACT:
A process for producing trehalose from inexpensive saccharide raw materials via an enzymic method uses stable enzymes with high productivity. The process includes incubating a saccharide raw material and an inorganic phosphoric acid and/or a salt thereof in the presence of phosphorylase to produce .alpha.-glucose 1-phosphate, and contacting the produced .alpha.-glucose 1-phosphate with glucose in the presence of a trehalose phosphorylase to produce trehalose. The isolation and purification of trehalose is easier in comparison to conventional fermentation methods.
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Kitamoto, Y., et al., ".alpha.-Glucose-l-phosphate formation by a novel trehalose phosphorylase from Flammulina velutipes," FEMS Microbiology Letters, 55 (1988) 147-150.
Murao et al., "Enzymatic Synthesis of Trehalose from Maltose," Agric. Biol. Chem., 49 (7), 2113-2118, 1985.
Takuro Koga et al., "Purification and Some Properties of Sucrose Phosphorylase From Leuconostoc mesenteroides", Agric. Biol. Chem., vol. 55(7), pp. 1805-1810, 1991.
Atsumi Kamogawa et al., "Purification and Properites of Maltose Phosphorylase From Lactobacillus brevis", Agr. Biol. Chem., vol. 37 (12), pp. 2813-2819, 1973.
I. Schick et al., "Coenzyme-Independent Enzymatic Synthesis of .alpha.,.alpha.-Trehalose", Biochem. Eng. --Stuttgart., Proc. Int. Symp. J., pp. 126-129, 2nd 1990 (Pub. 1991).
Konai Yutaka
Takahashi Eisaku
Wada Toshihiko
Kureha Chemical Industry Co. Ltd.
Lilling Herbert J.
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