Chemistry: molecular biology and microbiology – Animal cell – per se ; composition thereof; process of... – Culture medium – per se
Patent
1995-07-11
1997-11-11
Lankford, Jr., Leon B.
Chemistry: molecular biology and microbiology
Animal cell, per se ; composition thereof; process of...
Culture medium, per se
435383, 435384, 435404, C12N 500
Patent
active
056863071
ABSTRACT:
Methods and formulations are disclosed for the in vitro formation of a histologically complete human epidermis in a serum-free, companion cell or cell feeder layer-free, and organotypic matrix-free culture system commencing with the isolation and cultivation of a unique population of clonally-competent basal epidermal cells and ending with the formation of a functional, histologically complete, human squamous epithelium. The formation of a histologically complete human epidermis is accomplished in a serum-free medium, without companion-cells or feeder layer cells or any organotypic support using a multi-step process that is controlled by manipulating the growth and differentiation factors requisite to the sequential development of a usable, functional, and completely differentiated epidermis. The entire culture process can be accomplished in a relatively short time (3 to 4 weeks) with complete reproducibility and can supply copious amounts (2 to 3 square meters) of viable reformed human epidermis for a variety of experimental, clinical and commercial purposes where a histologically-complete living skin substitute is required.
REFERENCES:
patent: 4673649 (1987-06-01), Boyce et al.
patent: 4940666 (1990-07-01), Boyce et al.
patent: 5122469 (1992-06-01), Mather et al.
patent: 5232848 (1993-08-01), Wolfe et al.
patent: 5292655 (1994-03-01), Wille, Jr.
patent: 5326699 (1994-07-01), Torishima et al.
patent: 5328844 (1994-07-01), Moore
patent: 5529920 (1996-06-01), Cole et al.
The Sigma Cell Culture Catalogue, pp. 230-231, 1994.
Wilke, M.S. et al., Amer. J. of Pathol., vol. 131(1), pp. 171-181, Apr. 1988.
Nissley, P. et al., Growth and Differentiation of Cells in a Defined Environment, pp. 337-344, 1985.
Sigma Catalogue & Price List, pp. 58-59, 1994.
Miyazaki, M. et al., In Vitro Cellular & Developmental Biology, vol. 25(9), pp. 839-848, Sep. 1989.
Boisseau, A.M. et al., Journal of Dermatological Science, vol. 3(2), pp. 111-120, 1992.
Hy-Gene, Inc.
Lankford , Jr. Leon B.
Nickey Donald O.
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